1.Remove the swab from the swab packaging. Insert the sterile swab first trans nasally into the nasopharynx until you feel a slight resistance and rotate it 3 times. Repeat the same procedure with the other nostril. Open the extraction buffer tube. Put your swap into the tube and rotate the swab 10 times to elute the sample into the buffer. Break off the upper part of the swab with squeezing.2. Close the buffer tube, and gently flip and mix. Open the aluminum package and place the cassette in a flat, dry area. Break off the tip of the cap at the selected point. Add 3 drops of sample-extraction buffer mixture to the sample well and read results after 15 mins.